bicans, Aspergillus fumigatus and Cryptococcus

After the biologically inactive precipitate was removed, the filtrate was adjusted to pH 5.0 which resulted in the formation of dark red precipitate of crude pradimicin. This solid was stirred in a mixture of BuOH-MeOH-1% NaCl (3 :1 :4, pH 2.0). The organic layer was separated and extracted with alkaline water (pH 8.0). The aqueous layer was adjusted to pH 2.0 and subjected to column chromatography on Diaion HP-20 eluted with 60% aq Me2CO (pH 3.0). The red, active eluate was concentrated